This examine introduces a novel holomorphic marine fungal species, Halobyssothecium estuariae (Lentitheciaceae, Pleosporales), from lifeless Phragmites communis. The new species has semi-immersed, subglobose or ellipsoidal, papillate, conical ascomata, clavate to subcylindrical, quick pedicellate asci and 3-septate, fusoid to ellipsoidal ascospores with rounded ends, pale brown to darkish brown central cells and hyaline finish cells.
The asexual morph has multiseptate, filiform, intercalary, catenate, branched chlamydospores that resemble Xylomyces. The asexual morph of Keissleriella phragmiticola based mostly on mixed LSU, SSU, ITS and TEF1 sequence analyses is reported. The function of molecular identification in delineating cryptic species are additionally mentioned.
First report of Colletotrichum fructicola inflicting anthracnose on Pouteria campechiana in China
Pouteria campechiana (Kunth) Baehni (=Lucuma nervosa A. DC.) is a fruit crop planted in southern China (Gao et al. 2019). It is initially from Central America, and additionally grown there commercially in addition to in some American states (Fadzilah et al. 2018). In March 2019, a leaf spot illness was discovered on P. campechiana in Baoshan, Yunnan, China. Field surveys have been completed in a 0.06 ha orchard in Yunnan Province. Leaf spots have been discovered on 90% of six-year-old vegetation on this subject and have been noticed in different planting areas. The signs initially appeared as small, spherical, brown spots. As the illness developed, the heart of the lesions was sunken with a darkish brown border (Fig. 1).
Under extreme situations, some spots have been joined into bigger irregular spots, and even complete leaves died. The illness severity of completely different vegetation diversified, and some leaves confirmed solely a few brown spots whereas others confirmed many spots. Small fragments of diseased tissues (3×Three mm) have been disinfected in 75% ethanol for 10 s, 1% NaClO for 1 min, and rinsed 3 times in sterilized water.
Then, tissues have been positioned onto potato dextrose agar (PDA), and incubated at 25°C in the darkish for five days. Fungal isolates with comparable morphology have been persistently recovered from diseased tissues. The 25 colonies have been initially cottony, pale white to pale grey on the higher facet and greyish-green with black zonation on the underside of plates.
Conidia have been single-celled and hyaline, aseptate, straight, and cylindrical, with rounded ends (Fig. 1B). The size and width of 200 conidia have been measured for 2 consultant isolates, DHG-1 and DHG-2, and these averaged 14.48 × 5.59 μm and 14.92 × 5.57 μm. Appressoria have been ovoid, generally clavate, brown, averaged 7.47 × 5.86 μm and 7.25 × 5.85 μm (n=30). Brown and globose ascocarp have been noticed on the leaves of Pouteria campechiana.
Asci have been unitunicate, thin-walled, 6-Eight spored, clavate, averaged 51.53×13.01 μm and 50.21 × 13.32 μm (n=30). Ascospores have been hyaline, one-celled, barely curved to curved with obtuse to barely rounded ends, averaged 14.64×5.97 μm and 15.19 × 6.23 μm (n=30). These two isolates have been chosen for molecular identification. DNA was extracted from mycelia with the DNA safe Plant Kit (TIANGEN, Biotech, China).
For additional molecular identification, the inside transcribed spacer (ITS), partial actin (ACT), calmodulin (CAL), chitin synthase (CHS-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-tubulin (TUB2), and the Apn2-Mat1-2 intergenic spacer and partial mating kind (Mat1-2) gene (ApMat) genes of the strains (DHG-1, DHG-2) have been amplified utilizing the primer pairs ITS1/ITS4, ACT-512F/ACT-783R, CL1C/CL2C, CHS-79F/CHS-345R, GDF1/GDR1, T1/Bt-2b, and AM-F/AM-R (Weir et al. 2012; Silva et al. 2012), respectively.
The sequences have been obtained and in contrast with GenBank and all of them confirmed over 99% id to the kind pressure of Colletotrichum fructicola ICMP 18581 (Accession nos. JX010165, JX010033, JQ807838, FJ907426, JX010405, JX009866, and FJ917508) (Weir et al. 2012). A phylogenetic tree based mostly on the mixed ITS, ACT, CAL, CHS-1, TUB2, GAPDH and ApMat sequences utilizing the Neighbor-joining algorithm revealed that the isolates have been C. fructicola (Fig. 2). The sequences have been deposited into GenBank with accession MN955541, MN955542, and MN966581 to MN966592.
Pathogenicity assessments have been carried out on eighteen wholesome and tender leaves of six 1-year-old P. campechiana vegetation in a greenhouse. The experiment was repeated twice. The size and width of the inoculated leaves have been between 8-13 cm × 2.5-3.6 cm. The dermis of every examined leaf was evenly scratched in six separate areas with a sterilized needle. Each isolate was inoculated onto at the very least three wounded leaves by inserting 20 μL of a conidial suspension (106 conidia/mL) on the wound websites. Control leaves have been additionally wounded and inoculated with distilled water.
All the vegetation have been then sprayed with distilled water and lined with plastic luggage. After 10 days, preliminary signs appeared as round and deep yellow spots. After a few extra days, the spots grew to become brown, enlarged to as much as 4.Zero mm which was much like signs noticed in the subject, whereas controls remained symptomless. Koch’s postulates have been fulfilled by re-isolation of C. fructicola from diseased leaves, and identification confirmed by sequencing.
Colletotrichum fructicola has been related to anthracnose on mango, apple, pear and cassava (Oliveira et al. 2018). To our data, that is the first report of C. fructicola related to anthracnose of P. campechiana worldwide. These outcomes will present essential info for future epidemiological research and for administration of this illness.
FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum
Ascospores are the main inoculum in Fusarium graminearum, a causal agent of wheat head blight. In a earlier examine, FgPAL1 was discovered to be up-regulated in the Fgama1 mutant and vital for ascosporogenesis. However, the organic operate of this well-conserved gene in filamentous ascomycetes isn’t clear. In this examine, we characterised its capabilities in progress, differentiation, and pathogenesis. The Fgpal1 mutant had extreme progress defects and typically displayed irregular hyphal ideas.
It was faulty in infectious progress in rachis tissues and spreading in wheat heads. The Fgpal1 mutant produced conidia with fewer septa and extra nuclei per compartment than the wild kind. In actively rising hyphal ideas, FgPal1-GFP primarily localized to the subapical collar and septa. The FgPal1 and LifeAct partially co-localized at the subapical area in the interdependent method.
Tenascin C (TNC) Antibody |
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| 20-abx129349 | Abbexa |
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Tenascin C (TNC) Antibody |
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| 20-abx130202 | Abbexa |
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Tenascin-C Polyclonal Antibody |
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| ABP53279-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of Tenascin-C from Human, Mouse, Rat. This Tenascin-C antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tenascin-C |
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Tenascin-C Polyclonal Antibody |
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| ABP53279-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of Tenascin-C from Human, Mouse, Rat. This Tenascin-C antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tenascin-C |
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Tenascin-C Polyclonal Antibody |
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| ABP53279-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of Tenascin-C from Human, Mouse, Rat. This Tenascin-C antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Tenascin-C |
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Tenascin C (TNC) Antibody |
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| 20-abx100416 | Abbexa |
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Tenascin C (TNC) Antibody |
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| 20-abx100417 | Abbexa |
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Tenascin C (TNC) Antibody |
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| 20-abx100418 | Abbexa |
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Tenascin C (TNC) Antibody |
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| abx433386-200ul | Abbexa | 200 ul | EUR 286 |
Tenascin C (TNC) Antibody |
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| abx219024-100ug | Abbexa | 100 ug | EUR 439 |
Tenascin C (TNC) Antibody |
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| 20-abx212803 | Abbexa |
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Anti-Tenascin-C antibody |
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| STJ97243 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Rabbit polyclonal to Tenascin-C. |
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Anti-Tenascin-C antibody |
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| STJ16100797 | St John's Laboratory | 100 µg | EUR 557 |
Anti-Tenascin-C antibody |
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| STJ16100798 | St John's Laboratory | 100 µg | EUR 557 |
Anti-Tenascin-C antibody |
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| STJ16101005 | St John's Laboratory | 100 µg | EUR 354 |
Tenascin-C Polyclonal Antibody |
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| 41914-50ul | SAB | 50ul | EUR 187 |
Tenascin C (TNC) Antibody |
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| 20-abx327174 | Abbexa |
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Tenascin C Conjugated Antibody |
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| C48835 | SAB | 100ul | EUR 397 |
Tenascin-C Polyclonal Antibody |
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| 41914-100ul | SAB | 100ul | EUR 252 |
Tenascin (TN-C) Antibody |
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| 3628-100 | Biovision | EUR 349 | |
Tenascin (TN-C) Antibody |
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| 3628-30T | Biovision | EUR 146 | |
Anti-Tenascin C Antibody |
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| A1666-100 | Biovision | EUR 479 | |
anti-Tenascin C |
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| YF-PA23936 | Abfrontier | 50 ul | EUR 334 |
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Description: Mouse polyclonal to Tenascin C |
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Tenascin antibody |
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| 70R-15292 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rabbit polyclonal Tenascin antibody |
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Tenascin antibody |
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| 10R-7904 | Fitzgerald | 100 ug | EUR 322 |
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Description: Mouse monoclonal Tenascin antibody |
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Tenascin antibody |
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| 10R-T103a | Fitzgerald | 100 ug | EUR 476 |
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Description: Mouse monoclonal Tenascin antibody |
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Tenascin antibody |
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| 70R-14232 | Fitzgerald | 100 ug | EUR 322 |
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Description: Affinity purified Mouse polyclonal Tenascin antibody |
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Tenascin C (Stromal Marker For Epithelial Malignancy); Clone T2H5 (Concentrate) |
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| RA0135-C.1 | ScyTek Laboratories | 0.1 ml | EUR 125 |
Tenascin C (Stromal Marker For Epithelial Malignancy); Clone T2H5 (Concentrate) |
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| RA0135-C.5 | ScyTek Laboratories | 0.5 ml | EUR 247 |
Anti-Tenascin C/TNC Antibody |
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| PA2055 | BosterBio | 100ug/vial | EUR 334 |
Tenascin C (TNC) Antibody Pair |
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| abx117625-1pair5x96wellplates | Abbexa | 1 pair (5x96 well plates) | EUR 1010 |
Tenascin C (TNC) Antibody (Biotin) |
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| 20-abx272223 | Abbexa |
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Tenascin C (TNC) Antibody (Biotin) |
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| 20-abx273278 | Abbexa |
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Polyclonal Tenascin (TN-C) Antibody |
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| APR13724G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Tenascin (TN-C) . This antibody is tested and proven to work in the following applications: |
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Tenascin-C Polyclonal Conjugated Antibody |
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| C41914 | SAB | 100ul | EUR 397 |
Tenascin C Blocking Peptide |
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| 20-abx061883 | Abbexa |
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Tenascin C Blocking Peptide |
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| 20-abx062761 | Abbexa |
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Recombinant Tenascin C (TNC) |
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| 4-RPB975Hu01 | Cloud-Clone |
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Description: Recombinant Human Tenascin C expressed in: E.coli |
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Recombinant Tenascin C (TNC) |
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| 4-RPB975Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Tenascin C expressed in: E.coli |
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Recombinant Tenascin C (TNC) |
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| 4-RPB975Ra01 | Cloud-Clone |
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Description: Recombinant Rat Tenascin C expressed in: E.coli |
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Recombinant Tenascin C (TNC) |
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| 4-RPB975Ra02 | Cloud-Clone |
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Description: Recombinant Rat Tenascin C expressed in: E.coli |
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Recombinant Tenascin C (TNC) |
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| 4-RPB975Ra03 | Cloud-Clone |
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Description: Recombinant Rat Tenascin C expressed in: E.coli |
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Recombinant human Tenascin-C |
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| P1634 | FN Test | 100ug | Ask for price |
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Description: Recombinant protein for human Tenascin-C |
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Anti-Tenascin C (3B4) |
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| YF-MA13620 | Abfrontier | 100 ug | EUR 363 |
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Description: Mouse monoclonal to Tenascin C |
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Polyclonal TNC / Tenascin C Antibody (C-Terminus) |
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| APR13764G | Leading Biology | 0.05ml | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TNC / Tenascin C (C-Terminus). This antibody is tested and proven to work in the following applications: |
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Tenascin Antibody (Biotin) |
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| 20-abx105965 | Abbexa |
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Tenascin Antibody (Biotin) |
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| 20-abx105966 | Abbexa |
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Tenascin Antibody (FITC) |
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| 20-abx107380 | Abbexa |
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Tenascin Antibody (FITC) |
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| 20-abx107381 | Abbexa |
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Tenascin Antibody (HRP) |
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| 20-abx108800 | Abbexa |
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Tenascin Antibody (HRP) |
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| 20-abx108801 | Abbexa |
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Tenascin (TNC) Antibody |
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| 20-abx110255 | Abbexa |
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Tenascin (TNC) Antibody |
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| 20-abx110256 | Abbexa |
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Tenascin(T2H5) Antibody |
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| BNCA0392-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against Tenascin(T2H5), APC conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNCAP0392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNCAP0392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC810392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF680R conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC810392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF680R conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC880392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF488A conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC880392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF488A conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNCB0392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), Biotin conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNCB0392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), Biotin conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC550392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF555 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC550392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF555 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC430392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF543 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC430392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF543 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC470392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF647 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC470392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF647 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC940392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF594 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC940392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF594 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNCP0392-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against Tenascin(T2H5), PerCP conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC040392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF405S conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC040392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF405S conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC050392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF405M conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC050392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF405M conjugate, Concentration: 0.1mg/mL |
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Tenascin (TNC) Antibody |
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| abx411626-02mg | Abbexa | 0.2 mg | EUR 565 |
Tenascin-X Antibody |
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| abx238596-100ug | Abbexa | 100 ug | EUR 509 |
Tenascin (TNC) Antibody |
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| abx140268-01mg | Abbexa | 0.1 mg | EUR 384 |
Tenascin(T2H5) Antibody |
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| BNCR0392-250 | Biotium | 250uL | EUR 383 |
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Description: Primary antibody against Tenascin(T2H5), RPE conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNUB0392-100 | Biotium | 100uL | EUR 209 |
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Description: Primary antibody against Tenascin(T2H5), Concentration: 0.2mg/mL |
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Tenascin(T2H5) Antibody |
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| BNUB0392-500 | Biotium | 500uL | EUR 458 |
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Description: Primary antibody against Tenascin(T2H5), Concentration: 0.2mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC680392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF568 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC680392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF568 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC700392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF770 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC700392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF770 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC800392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF680 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC800392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF680 conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC400392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF640R conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC400392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF640R conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC610392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), CF660R conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNC610392-500 | Biotium | 500uL | EUR 544 |
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Description: Primary antibody against Tenascin(T2H5), CF660R conjugate, Concentration: 0.1mg/mL |
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Tenascin(T2H5) Antibody |
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| BNCH0392-100 | Biotium | 100uL | EUR 199 |
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Description: Primary antibody against Tenascin(T2H5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
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The Fgpal1 mutant was regular in meiosis with eight nuclei in creating asci however most asci have been aborted. Taken collectively, our outcomes confirmed that FgPal1 performs a function in sustaining polarized tip progress and coordination between nuclear division and cytokinesis, and additionally it is vital for infectious progress and developments of ascospores by the free cell formation course of. This article is protected by copyright. All rights reserved.