Category: Vector & Virus

First Report of Erysiphe palczewskii Powdery Mildew of Siberian Pea Tree (Caragana arborescens) in Wisconsin and Quebec.

Shoots affected by powdery mildew had been collected from Siberian pea bushes in July 2009 on the University of Wisconsin-Madison campus and on the campus of Université Laval, Quebec City, Quebec. This unique shrub or small tree is occasionally planted in Wisconsin and three shrubs in a bunch that had been affected are the one examples identified on the UW-Madison campus.
In Quebec City, Siberian pea tree is extra generally used as a decorative, usually in hedges (as is the case of the affected vegetation on the Université Laval campus). In each areas, <10% of foliage was visibly affected, however incidence was better on shoots nearer to the bottom than on increased shoots. White-to-grayish mycelium was current on leaves and younger stems and generally utterly lined each higher and decrease leaf surfaces. Dark brown-to-black chasmothecia had been quite a few on leaf blades, petioles, and younger stems, however had been most plentiful on decrease surfaces of leaves.
Morphology of chasmothecia, together with appendages with distinctive terminal dichotomous branching, (1) was in line with descriptions and illustrations of the fungus Erysiphe palczewskii Jacz. (synonym Microsphaera palczewskii) (1-4) considered native to Asia, however often known as an invader of Europe the place it happens on the identical host. For a pattern from Université Laval, imply diameter of chasmothecia was 113 μm, imply appendage size was 185 μm, and barrel-shaped conidia that lacked fibrosin our bodies averaged 30 × 14 μm. Asci contained oval, yellow ascospores with imply dimensions of 20 × 12 μm.
DNA was extracted from chasmothecia, and nuclear rDNA sequences (633 nucleotides) of the Wisconsin (GenBank Accession No. GQ497277) and Quebec (GenBank Accession No. GQ497276) specimens differed by just one nucleotide. The sequences that had been obtained most intently matched GenBank sequences for Oidium spp. (98%) and Erysiphe spp. (97%). Further observations indicated that the identical pathogen affected Siberian pea bushes planted as ornamentals at a number of areas separated by ≥15 km in the metropolitan Quebec space.
This report extends the japanese identified restrict of E. palczewskii in the United States, beforehand identified from collections in Alaska (2), Washington (4), Idaho (4), North Dakota (3), and Minnesota (3). To our information, that is the primary report of this illness in Canada, and it signifies that the distribution of E. palczewskii is transcontinental.
Specimens from Madison, WI and Quebec, QC have been deposited in the U.S. National Fungus Collections (BPI 879152) and the Rene Pomerleau Herbarium of the Canadian Forest Service Laurentian Forestry Centre (QFB-22601). References: (1) U. Braun. Beih. Nova Hedwigia 89:1, 1987. (2) D. A. Glawe and G. A. Laursen. Online publication. doi:10:1094/PHP-2005-1017-01-BR. Plant Health Progress, 2005. (3) D. A. Glawe et al. Online publication. doi:10.1094/PHP-2006-0117-01-BR. Plant Health Progress, 2006. (4) C. Nischwitz and G. Newcombe. Plant Dis. 87:451, 2003.

First Report of Powdery Mildew Caused by Golovinomyces biocellatus on Peppermint in California.

In August of 2009, powdery mildew was noticed on peppermint (Mentha piperita L.) in a number of industrial fields in the Fall River Valley of japanese Shasta County, California. Plant progress was apparently diminished by the illness, however its affect on yield was unknown. White fungal progress was restricted to the adaxial surfaces, the place colonies had been skinny and effused. Heavily contaminated leaves developed a reddish tint as progress prematurely ceased. Doliform conidia ([26.6-] 29.2 [-31.7] × [13.2-] 15.6 [-16.8] μm) had been produced in chains of roughly six conidia.
Foot cells had been cylindrical ([41.3-] 55.2 [-75.0] × [11.2-] 12.0 [-12.8] μm). Immature chasmothecia had been yellowish brown and roughly 100.Zero μm in diameter with flexuous, mycelium-like appendages as much as 200 μm lengthy. All these options had been in line with these of Golovinomyces biocellatus. Asci weren’t noticed. To affirm the identification of the fungus, nuclear rDNA inside transcribed spacer (ITS) areas had been amplified by PCR with common primers ITS4 and ITS5.
The sequence (537 bp) was a precise match for a number of submissions of G. biocellatus in GenBank (e.g., Accession No. EU035602, a sequence of the fungus from mint in Australia [1]). Pathogenicity was confirmed by brushing spores from naturally contaminated leaves onto three rooted cuttings of M. piperita ‘Black Mitchum’. After the vegetation had been lined with a plastic bag for 36 h to keep up excessive humidity, they had been stored on a greenhouse bench at 23 to 28°C.
Three noninoculated vegetation, which served as controls, had been positioned in one other greenhouse in related situations. The experiment was repeated as soon as. All inoculated vegetation developed indicators of powdery mildew inside 7 days of inoculation whereas noninoculated vegetation remained illness free. The fungus on inoculated leaves was morphologically indistinguishable from the one used to inoculate the vegetation. To our information, that is the primary report of G. biocellatus on peppermint in California. References: (1) J. R. Liberato and J. H. Cunnington. Australas, Plant Dis. Notes 2:38, 2007.

Root and Crown Rot of Anthurium Caused by Calonectria ilicicola in Iran.

In the autumn of 2008, a extreme illness of Anthurium andraeanum with wilting and root and crown rot signs was noticed in a greenhouse in the Varamin space of Tehran. A species of Calonectria was remoted constantly from symptomatic tissues on 2% potato dextrose agar (PDA). The fungus produced perithecia and a Cylindrocladium anamorph when incubated on carnation leaf agar beneath near-ultraviolet mild at 25°C. Perithechia had been reddish brown, subglobose to ovoid, and 300 to 400 μm in diameter. Asci had been clavate, hyaline, 90 to 140 × 12 to 19 μm, and tapering to a protracted skinny stalk.
First Report of Erysiphe palczewskii Powdery Mildew of Siberian Pea Tree (Caragana arborescens) in Wisconsin and Quebec.
Ascospores had been fusoid, straight to barely curved, 1- (-3) septate, and (30-) 37 to 50 (-65) × (4-) 5 to six.5 (-7) μm (imply = 45 × 6 μm; n = 30). Penicillate conidiophores gave rise to stipe extensions that terminated in sphaeropedunculate vesicles (6-) 7 to 10 (-12) μm in diameter. Conidia had been hyaline, cylindrical, rounded at each ends, straight, (45-) 70 to 82 (-90) × (4-) 5 to six.5(-7) μm (imply = 62 × 6 μm; n = 30), and (1-) 3-septate. On the premise of morphology, the fungus was recognized as Calonectria ilicicola Boedijin & Reitsma.
Koch’s postulates had been fulfilled by spray inoculating 1-month-old seedlings with a conidial and mycelial suspension (105 particles per ml) of the fungus obtained from 14-day-old single-spore colonies grown on PDA at 25°C. Following inoculation, all vegetation had been maintained in plastic baggage in a glasshouse at 25 ± 1°C. After 15 to 25 days, signs resembling these seen in the diseased glasshouse had been detected on inoculated vegetation. C. ilicicola was reisolated from the artificially contaminated tissues.
No signs had been detected on the management vegetation. Nucleotide sequences of the interior transcribed spacer (ITS) areas of the nrDNA operon and the partial histone H3 gene had been decided for derived pressure CPC 16334 as described beforehand (1,3). The ITS sequence (GenBank Accession No. GU057378) matched 100% (644/644 bp) with the sequence of C. ilicicola pressure CBS 463.76 (GenBank AF493963) and the histone H3 sequence (GenBank GU057379) matched 99% (456/458 bp; as a result of two versus three AC repeats in the sequence) with that of C.

Neurofascin (NFASC) Antibody
abx431852-200ul 200 ul
EUR 384

Neurofascin (NFASC) Antibody
20-abx241687
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Neurofascin (NFASC) Antibody
20-abx214122
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Neurofascin (NFASC) Antibody
20-abx173762
  • EUR 926.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Recombinant Neurofascin (NFASC)
4-RPL939Hu01
  • EUR 510.37
  • EUR 239.00
  • EUR 1638.88
  • EUR 612.96
  • EUR 1125.92
  • EUR 404.00
  • EUR 3947.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Neurofascin expressed in: E.coli

Human Neurofascin (NFASC)
1-CSB-EP015743HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Neurofascin(NFASC),partial expressed in E.coli

Human Neurofascin (NFASC) Protein
20-abx068240
  • EUR 718.00
  • EUR 286.00
  • EUR 2207.00
  • EUR 843.00
  • EUR 509.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Neurofascin (NFASC) Polyclonal Antibody (Human)
4-PAL939Hu01
  • EUR 262.00
  • EUR 2747.00
  • EUR 679.00
  • EUR 331.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC)

Chicken Neurofascin, NFASC ELISA KIT
ELI-15003c 96 Tests
EUR 928

Human Neurofascin, NFASC ELISA KIT
ELI-20708h 96 Tests
EUR 824

Mouse Neurofascin, Nfasc ELISA KIT
ELI-45737m 96 Tests
EUR 865

Human Neurofascin (NFASC) ELISA Kit
SEL939Hu-10x96wellstestplate 10x96-wells test plate
EUR 5189.65
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids.

Human Neurofascin (NFASC) ELISA Kit
SEL939Hu-1x48wellstestplate 1x48-wells test plate
EUR 515.03
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids.

Human Neurofascin (NFASC) ELISA Kit
SEL939Hu-1x96wellstestplate 1x96-wells test plate
EUR 692.9
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids.

Human Neurofascin (NFASC) ELISA Kit
SEL939Hu-5x96wellstestplate 5x96-wells test plate
EUR 2818.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids.

Human Neurofascin (NFASC) ELISA Kit
4-SEL939Hu
  • EUR 5240.00
  • EUR 2769.00
  • EUR 693.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neurofascin (NFASC) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Neurofascin(NFASC) ELISA kit
CSB-EL015743HU-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Neurofascin (NFASC) in samples from serum, plasma, cerebrospinalfluid (CSF), tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Neurofascin(NFASC) ELISA kit
1-CSB-EL015743HU
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Neurofascin(NFASC) in samples from serum, plasma, cerebrospinalfluid(CSF), tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Neurofascin (NFASC) ELISA Kit
20-abx152492
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Neurofascin (NFASC) CLIA Kit
20-abx495996
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Neurofascin (NFASC) ELISA Kit
abx390014-96tests 96 tests
EUR 911

Rat Neurofascin (NFASC) ELISA Kit
abx391685-96tests 96 tests
EUR 911

Human Neurofascin(NFASC)ELISA Kit
QY-E01656 96T
EUR 394

Neurofascin (NFASC) Polyclonal Antibody (Human), APC
4-PAL939Hu01-APC
  • EUR 368.00
  • EUR 3599.00
  • EUR 993.00
  • EUR 472.00
  • EUR 229.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with APC.

Neurofascin (NFASC) Polyclonal Antibody (Human), Biotinylated
4-PAL939Hu01-Biotin
  • EUR 328.00
  • EUR 2697.00
  • EUR 786.00
  • EUR 404.00
  • EUR 226.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with Biotin.

Neurofascin (NFASC) Polyclonal Antibody (Human), Cy3
4-PAL939Hu01-Cy3
  • EUR 449.00
  • EUR 4757.00
  • EUR 1283.00
  • EUR 588.00
  • EUR 264.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with Cy3.

Neurofascin (NFASC) Polyclonal Antibody (Human), FITC
4-PAL939Hu01-FITC
  • EUR 314.00
  • EUR 2899.00
  • EUR 814.00
  • EUR 397.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with FITC.

Neurofascin (NFASC) Polyclonal Antibody (Human), HRP
4-PAL939Hu01-HRP
  • EUR 335.00
  • EUR 3135.00
  • EUR 877.00
  • EUR 426.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with HRP.

Neurofascin (NFASC) Polyclonal Antibody (Human), PE
4-PAL939Hu01-PE
  • EUR 314.00
  • EUR 2899.00
  • EUR 814.00
  • EUR 397.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with PE.

ELISA kit for Human NFASC (Neurofascin)
ELK6104 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Neurofascin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Neurofascin (NFASC) Polyclonal Antibody (Human), APC-Cy7
4-PAL939Hu01-APC-Cy7
  • EUR 616.00
  • EUR 7078.00
  • EUR 1867.00
  • EUR 824.00
  • EUR 338.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with APC-Cy7.

Rabbit Anti- Rat neurofascin (Nfasc)-phospor IgG (aff pure)
AB-23249-A 100ug
EUR 482

Nfasc ELISA Kit| Rat Neurofascin ELISA Kit
EF019045 96 Tests
EUR 689

Nfasc ELISA Kit| Mouse Neurofascin ELISA Kit
EF015653 96 Tests
EUR 689

NFASC ELISA Kit| chicken Neurofascin ELISA Kit
EF012420 96 Tests
EUR 689

Rat neurofascin (Nfasc)-control Control/blocking peptide
AB-23249-CP 100ug
EUR 164

Rat neurofascin (Nfasc)-phosphor Control/blocking peptide
AB-23249-P 100ug
EUR 164

Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-100ug
QP6416-ec-100ug 100ug
EUR 408

Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-10ug
QP6416-ec-10ug 10ug
EUR 200

Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-1mg
QP6416-ec-1mg 1mg
EUR 1632

Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-200ug
QP6416-ec-200ug 200ug
EUR 634

Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-500ug
QP6416-ec-500ug 500ug
EUR 1060

Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-50ug
QP6416-ec-50ug 50ug
EUR 263

Anti-NFASC antibody
STJ24750 100 µl
EUR 277
Description: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349

Anti-Neurofascin (aa877-890) antibody
STJ72603 100 µg
EUR 359

Neurofascin
PR15066 50 ug
EUR 468

NFASC Antibody
ABD7160 100 ug
EUR 438

NFASC Antibody
DF7160 200ul
EUR 304
Description: NFASC Antibody detects endogenous levels of total NFASC.

NFASC Antibody
1-CSB-PA073153
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against NFASC. Recognizes NFASC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

NFASC Antibody
1-CSB-PA787178
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against NFASC. Recognizes NFASC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

NFASC Antibody
31250-100ul 100ul
EUR 252

NFASC Antibody
31250-50ul 50ul
EUR 187

NFASC antibody
38553-100ul 100ul
EUR 252

Nfasc/ Rat Nfasc ELISA Kit
ELI-22181r 96 Tests
EUR 886

NFASC Conjugated Antibody
C38553 100ul
EUR 397

NFASC Conjugated Antibody
C31250 100ul
EUR 397

NFASC siRNA
20-abx925794
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

NFASC siRNA
20-abx925795
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

NFASC siRNA
20-abx903541
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280

NFASC Blocking Peptide
DF7160-BP 1mg
EUR 195

NFASC Rabbit pAb
A3053-100ul 100 ul
EUR 308

NFASC Rabbit pAb
A3053-200ul 200 ul
EUR 459

NFASC Rabbit pAb
A3053-20ul 20 ul
EUR 183

NFASC Rabbit pAb
A3053-50ul 50 ul
EUR 223

Polyclonal Neurofascin (aa877-890) Antibody (internal region)
AMM06631G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Neurofascin (aa877-890) (internal region). This antibody is tested and proven to work in the following applications:

NFASC ELISA KIT|Human
EF005338 96 Tests
EUR 689

Rat NFASC shRNA Plasmid
20-abx987343
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse NFASC shRNA Plasmid
20-abx983017
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human NFASC shRNA Plasmid
20-abx958008
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Nfasc ORF Vector (Rat) (pORF)
ORF071257 1.0 ug DNA
EUR 506

Nfasc ORF Vector (Rat) (pORF)
ORF071258 1.0 ug DNA
EUR 506

Nfasc ORF Vector (Rat) (pORF)
ORF071259 1.0 ug DNA
EUR 506

Nfasc ORF Vector (Rat) (pORF)
ORF071260 1.0 ug DNA
EUR 506

NFASC ORF Vector (Human) (pORF)
ORF026068 1.0 ug DNA
EUR 405

Nfasc ORF Vector (Mouse) (pORF)
ORF051272 1.0 ug DNA
EUR 506

Nfasc ORF Vector (Mouse) (pORF)
ORF051273 1.0 ug DNA
EUR 506

Nfasc ORF Vector (Mouse) (pORF)
ORF051274 1.0 ug DNA
EUR 506

Nfasc ORF Vector (Mouse) (pORF)
ORF051275 1.0 ug DNA
EUR 506

NFASC ELISA Kit (Human) (OKCD01074)
OKCD01074 96 Wells
EUR 909
Description: Description of target: Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.By similarity ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.115 ng/mL

Nfasc sgRNA CRISPR Lentivector set (Rat)
K7550101 3 x 1.0 ug
EUR 339

Nfasc sgRNA CRISPR Lentivector set (Mouse)
K4184901 3 x 1.0 ug
EUR 339

NFASC sgRNA CRISPR Lentivector set (Human)
K1420901 3 x 1.0 ug
EUR 339

NFASC 3'UTR Luciferase Stable Cell Line
TU015618 1.0 ml
EUR 4617

Nfasc 3'UTR Luciferase Stable Cell Line
TU114025 1.0 ml Ask for price

Nfasc 3'UTR GFP Stable Cell Line
TU164025 1.0 ml Ask for price

Nfasc 3'UTR Luciferase Stable Cell Line
TU213920 1.0 ml Ask for price

Nfasc 3'UTR GFP Stable Cell Line
TU263920 1.0 ml Ask for price

NFASC 3'UTR GFP Stable Cell Line
TU065618 1.0 ml
EUR 4617

NFASC Protein Vector (Human) (pPB-C-His)
PV104270 500 ng
EUR 552

NFASC Protein Vector (Human) (pPB-N-His)
PV104271 500 ng
EUR 552

NFASC Protein Vector (Human) (pPM-C-HA)
PV104272 500 ng
EUR 552

NFASC Protein Vector (Human) (pPM-C-His)
PV104273 500 ng
EUR 552

NFASC Protein Vector (Mouse) (pPB-C-His)
PV205086 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-N-His)
PV205087 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-HA)
PV205088 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-His)
PV205089 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-C-His)
PV205090 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-N-His)
PV205091 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-HA)
PV205092 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-His)
PV205093 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-C-His)
PV205094 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-N-His)
PV205095 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-HA)
PV205096 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-His)
PV205097 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-C-His)
PV205098 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPB-N-His)
PV205099 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-HA)
PV205100 500 ng
EUR 1065

NFASC Protein Vector (Mouse) (pPM-C-His)
PV205101 500 ng
EUR 1065

NFASC Protein Vector (Rat) (pPB-C-His)
PV285026 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPB-N-His)
PV285027 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPM-C-HA)
PV285028 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPM-C-His)
PV285029 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPB-C-His)
PV285030 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPB-N-His)
PV285031 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPM-C-HA)
PV285032 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPM-C-His)
PV285033 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPB-C-His)
PV285034 500 ng
EUR 1191

NFASC Protein Vector (Rat) (pPB-N-His)
PV285035 500 ng
EUR 1191
ilicicola pressure CBS 112217 (GenBank AY725686). To our information, that is the primary report of Calonectria and Cylindrocladium genera and the illness brought on by C. ilicicola from Iran. References: (1) R. Cheewangkoon et al. Persoonia 23:55, 2009. (2) P. W. Crous and M. J. Wingfield. Mycotaxon 51:341, 1994. (3) P. W. Crous et al. Stud. Mycol. 50:415, 2004.

miR-495 reduces neuronal cell apoptosis and relieves acute spinal cord injury through inhibiting PRDM5

This research goals to research the position of miR-495 in neuronal cell apoptosis after acute spinal cord injury (ASCI). The ASCI rat mannequin was established and the Basso, Beattie, and Bresnahan (BBB) rating was assessed. miR-495, PR area containing 5 (PRDM5), and Bcl-2 expressions have been measured by qRT-PCR or western blotting. Neuronal cell line PC-12 was subjected to hypoxia situation to simulate the in vitro ASCI mannequin. PC-12 cell apoptosis was measured by movement cytometry, and the interplay between miR-495 and PRDM5 was confirmed by twin luciferase reporter assay.

Results confirmed that BBB rating was considerably decreased in ASCI rats in contrast with sham rats. miR-495 expression was down-regulated in spinal cord tissue of ASCI rats and hypoxia-induced PC-12 cells, and PRDM5 protein stage was up-regulated in spinal cord tissue of ASCI rats and hypoxia-induced PC-12 cells. miR-495 overexpression may cut back apoptosis of PC-12 cells, and up-regulated anti-apoptosis protein Bcl-2 protein stage.

Moreover, PRDM5 was a goal of miR-495, and mRNA and protein ranges of PRDM5 have been negatively regulated by miR-495. miR-495 overexpression may cut back the hypoxia-induced PC-12 cell apoptosis, whereas PRDM5 overexpression abolished this inhibiting impact. The agomir-495 was injected into ASCI rats, and Bcl-2 protein stage and BBB rating have been elevated, however the PRDM5 overexpression reversed these outcomes. Overall, we concluded that miR-495 may inhibit neuronal cell apoptosis and relieve acute spinal cord injury through inhibiting PRDM5.

Second-Order Orbital Optimization with Large Active Spaces Using Adaptive Sampling Configuration Interaction (ASCI) and Its Application to Molecular Geometry Optimization

Recently, chosen configuration interplay (SCI) strategies that allow calculations with a number of tens of lively orbitals have been developed. With the SCI subspace embedded within the imply area, molecular orbitals with an accuracy akin to that of the whole lively area self-consistent area methodology will be obtained. Here, we implement the analytical gradient concept for the single-state adaptive sampling CI (ASCI) SCF methodology to allow molecular geometry optimization.
The ensuing analytical gradient is inherently approximate because of the dependence on the sampled determinants, however its accuracy was adequate for performing geometry optimizations with giant lively areas. To acquire the tight convergence wanted for correct analytical gradients, we mix the augmented Hessian (AH) and Werner-Meyer-Knowles (WMK) second-order orbital optimization strategies with the ASCI-SCF methodology. We take a look at these algorithms for orbital and geometry optimizations, display functions of the geometry optimizations of polyacenes and periacenes, and talk about the geometric dependence of the traits of singlet ASCI wave features.

First report of Erysiphe corylacearum, agent of powdery mildew, on hazelnut ( Corylus avellana) in Romania

Romania has an space devoted to hazelnut (Corylus avellana L.), protecting 890 hectares as of 2019. During October 2020, powdery mildew signs have been noticed on the higher facet of leaves of hazelnut ‘Tonda di Giffoni’ in two business orchards in Dudeștii Vechi, Romania (Fig. 1). The illness was current on 70% of the bushes in planting, with no less than 5 leaves per tree having powdery mildew. Micromorphological examination revealed amphigenous, hyaline, branched, septate mycelial patches of two.
Three to three.6 μm in diameter. Conidiophores measured 24-60 × 5-6 (common: 45 × 6) μm and consisted of erect, cylindrical to flexuous foot cells, adopted by 1-2 shorter cells. Ellipsoid, ovoid to doliform conidia have been produced singly and they measured 19-35 × 16-24 (common: 28 × 19) μm. Chasmothecia have been spherical, 75 to 107 (common: 88) μm in diameter. Nine to 13 straight, generally flexuous, appendages measured 54 to 92 (common: 66) μm in size and that they had 5 occasions dichotomous branched apices with curved suggestions (Fig. 2). Each chasmothecium contained three to 5 ellipsoid, ovoid to subglobose asci measuring 41-58 × 29-55 μm (common 52 × 43) μm.
miR-495 reduces neuronal cell apoptosis and relieves acute spinal cord injury through inhibiting PRDM5
The asci contained 4 to eight ascospores measuring 13-24 × 11-15 (common 18 × 14) μm. Morphological identification was confirmed by sequencing the ITS-region of rDNA utilizing two isolates from leaves, saved as frozen mycelium at -20°C. PCR was carried out with Erysiphales-specific primer pair PMITS1/PMITS2 (Cunnington et al. 2003). The obtained sequences have been deposited in GenBank (Accession n° MW423075, MW423076).
Blast evaluation of each sequences had 100% identification to ITS rDNA sequences of Erysiphe corylacearum from Azerbaijan (Abasova et al. 2018; Accession n° LC270863), Turkey (Sezer et al. 2017; KY082910), Switzerland (Beenken et al. 2020; MN82272), Iran (Arzanlou et al. 2018; MH047243), Italy (Mezzalama et al. 2020; MW045425) and 99% identification from Georgia (Meparishvili et al. 2019; MK157199).
The sequences had a decrease % identification (83%) to Phyllactinia guttata (Accession n° AB080558) (Fig. 3). Pathogenicity was verified on one-year-old vegetation of C. avellana ‘Tonda di Giffoni’, which have been artificially inoculated with a conidial suspension from contaminated leaves (n = 25). Inoculated vegetation have been incubated at 20 to 28°C with 70 to 80% relative humidity.
White mycelium appeared on the higher floor of the leaves at eight to 10 days after inoculation. No signs have been discovered on management vegetation sprayed with sterile water. The fungus current on inoculated leaves was morphologically an identical to the unique isolates from diseased bushes from the sector. E. corylacearum is native to East Asia and was beforehand reported in Japan on wild species of Corylus (Takamatsu et al. 2015; Accession n° LC009928).
The pathogen more than likely unfold into Europe from east to west of Europe (Heluta et al. 2019), through the Caucasus, ranging from Turkey, Azerbaijan, Georgia, and Iran. P. guttata was thought of the one causal agent of powdery mildew on hazelnut in most international locations, together with Romania (Brown 1995). Compared to P. guttata, which typically develops a mycelium on the underside of leaves, E. corylacearum grows with a white mycelium on the higher facet of the leaves.

TNN Antibody, FITC conjugated
1-CSB-PA892498LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against TNN. Recognizes TNN from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

TNN Antibody, Biotin conjugated
1-CSB-PA892498LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against TNN. Recognizes TNN from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Tenascin-N (TNN) Antibody
20-abx213586
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Tenascin-N (TNN) Antibody
20-abx213587
  • EUR 411.00
  • EUR