Shoots affected by powdery mildew had been collected from Siberian pea bushes in July 2009 on the University of Wisconsin-Madison campus and on the campus of Université Laval, Quebec City, Quebec. This unique shrub or small tree is occasionally planted in Wisconsin and three shrubs in a bunch that had been affected are the one examples identified on the UW-Madison campus.
In Quebec City, Siberian pea tree is extra generally used as a decorative, usually in hedges (as is the case of the affected vegetation on the Université Laval campus). In each areas, <10% of foliage was visibly affected, however incidence was better on shoots nearer to the bottom than on increased shoots. White-to-grayish mycelium was current on leaves and younger stems and generally utterly lined each higher and decrease leaf surfaces. Dark brown-to-black chasmothecia had been quite a few on leaf blades, petioles, and younger stems, however had been most plentiful on decrease surfaces of leaves.
Morphology of chasmothecia, together with appendages with distinctive terminal dichotomous branching, (1) was in line with descriptions and illustrations of the fungus Erysiphe palczewskii Jacz. (synonym Microsphaera palczewskii) (1-4) considered native to Asia, however often known as an invader of Europe the place it happens on the identical host. For a pattern from Université Laval, imply diameter of chasmothecia was 113 μm, imply appendage size was 185 μm, and barrel-shaped conidia that lacked fibrosin our bodies averaged 30 × 14 μm. Asci contained oval, yellow ascospores with imply dimensions of 20 × 12 μm.
DNA was extracted from chasmothecia, and nuclear rDNA sequences (633 nucleotides) of the Wisconsin (GenBank Accession No. GQ497277) and Quebec (GenBank Accession No. GQ497276) specimens differed by just one nucleotide. The sequences that had been obtained most intently matched GenBank sequences for Oidium spp. (98%) and Erysiphe spp. (97%). Further observations indicated that the identical pathogen affected Siberian pea bushes planted as ornamentals at a number of areas separated by ≥15 km in the metropolitan Quebec space.
This report extends the japanese identified restrict of E. palczewskii in the United States, beforehand identified from collections in Alaska (2), Washington (4), Idaho (4), North Dakota (3), and Minnesota (3). To our information, that is the primary report of this illness in Canada, and it signifies that the distribution of E. palczewskii is transcontinental.
Specimens from Madison, WI and Quebec, QC have been deposited in the U.S. National Fungus Collections (BPI 879152) and the Rene Pomerleau Herbarium of the Canadian Forest Service Laurentian Forestry Centre (QFB-22601). References: (1) U. Braun. Beih. Nova Hedwigia 89:1, 1987. (2) D. A. Glawe and G. A. Laursen. Online publication. doi:10:1094/PHP-2005-1017-01-BR. Plant Health Progress, 2005. (3) D. A. Glawe et al. Online publication. doi:10.1094/PHP-2006-0117-01-BR. Plant Health Progress, 2006. (4) C. Nischwitz and G. Newcombe. Plant Dis. 87:451, 2003.
First Report of Powdery Mildew Caused by Golovinomyces biocellatus on Peppermint in California.
In August of 2009, powdery mildew was noticed on peppermint (Mentha piperita L.) in a number of industrial fields in the Fall River Valley of japanese Shasta County, California. Plant progress was apparently diminished by the illness, however its affect on yield was unknown. White fungal progress was restricted to the adaxial surfaces, the place colonies had been skinny and effused. Heavily contaminated leaves developed a reddish tint as progress prematurely ceased. Doliform conidia ([26.6-] 29.2 [-31.7] × [13.2-] 15.6 [-16.8] μm) had been produced in chains of roughly six conidia.
Foot cells had been cylindrical ([41.3-] 55.2 [-75.0] × [11.2-] 12.0 [-12.8] μm). Immature chasmothecia had been yellowish brown and roughly 100.Zero μm in diameter with flexuous, mycelium-like appendages as much as 200 μm lengthy. All these options had been in line with these of Golovinomyces biocellatus. Asci weren’t noticed. To affirm the identification of the fungus, nuclear rDNA inside transcribed spacer (ITS) areas had been amplified by PCR with common primers ITS4 and ITS5.
The sequence (537 bp) was a precise match for a number of submissions of G. biocellatus in GenBank (e.g., Accession No. EU035602, a sequence of the fungus from mint in Australia [1]). Pathogenicity was confirmed by brushing spores from naturally contaminated leaves onto three rooted cuttings of M. piperita ‘Black Mitchum’. After the vegetation had been lined with a plastic bag for 36 h to keep up excessive humidity, they had been stored on a greenhouse bench at 23 to 28°C.
Three noninoculated vegetation, which served as controls, had been positioned in one other greenhouse in related situations. The experiment was repeated as soon as. All inoculated vegetation developed indicators of powdery mildew inside 7 days of inoculation whereas noninoculated vegetation remained illness free. The fungus on inoculated leaves was morphologically indistinguishable from the one used to inoculate the vegetation. To our information, that is the primary report of G. biocellatus on peppermint in California. References: (1) J. R. Liberato and J. H. Cunnington. Australas, Plant Dis. Notes 2:38, 2007.
Root and Crown Rot of Anthurium Caused by Calonectria ilicicola in Iran.
In the autumn of 2008, a extreme illness of Anthurium andraeanum with wilting and root and crown rot signs was noticed in a greenhouse in the Varamin space of Tehran. A species of Calonectria was remoted constantly from symptomatic tissues on 2% potato dextrose agar (PDA). The fungus produced perithecia and a Cylindrocladium anamorph when incubated on carnation leaf agar beneath near-ultraviolet mild at 25°C. Perithechia had been reddish brown, subglobose to ovoid, and 300 to 400 μm in diameter. Asci had been clavate, hyaline, 90 to 140 × 12 to 19 μm, and tapering to a protracted skinny stalk.
Ascospores had been fusoid, straight to barely curved, 1- (-3) septate, and (30-) 37 to 50 (-65) × (4-) 5 to six.5 (-7) μm (imply = 45 × 6 μm; n = 30). Penicillate conidiophores gave rise to stipe extensions that terminated in sphaeropedunculate vesicles (6-) 7 to 10 (-12) μm in diameter. Conidia had been hyaline, cylindrical, rounded at each ends, straight, (45-) 70 to 82 (-90) × (4-) 5 to six.5(-7) μm (imply = 62 × 6 μm; n = 30), and (1-) 3-septate. On the premise of morphology, the fungus was recognized as Calonectria ilicicola Boedijin & Reitsma.
Koch’s postulates had been fulfilled by spray inoculating 1-month-old seedlings with a conidial and mycelial suspension (105 particles per ml) of the fungus obtained from 14-day-old single-spore colonies grown on PDA at 25°C. Following inoculation, all vegetation had been maintained in plastic baggage in a glasshouse at 25 ± 1°C. After 15 to 25 days, signs resembling these seen in the diseased glasshouse had been detected on inoculated vegetation. C. ilicicola was reisolated from the artificially contaminated tissues.
No signs had been detected on the management vegetation. Nucleotide sequences of the interior transcribed spacer (ITS) areas of the nrDNA operon and the partial histone H3 gene had been decided for derived pressure CPC 16334 as described beforehand (1,3). The ITS sequence (GenBank Accession No. GU057378) matched 100% (644/644 bp) with the sequence of C. ilicicola pressure CBS 463.76 (GenBank AF493963) and the histone H3 sequence (GenBank GU057379) matched 99% (456/458 bp; as a result of two versus three AC repeats in the sequence) with that of C.
Neurofascin (NFASC) Antibody |
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| abx431852-200ul | Abbexa | 200 ul | EUR 384 |
Neurofascin (NFASC) Antibody |
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| 20-abx241687 | Abbexa |
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Neurofascin (NFASC) Antibody |
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| 20-abx214122 | Abbexa |
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Neurofascin (NFASC) Antibody |
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| 20-abx173762 | Abbexa |
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Human Neurofascin (NFASC) |
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| 1-CSB-EP015743HU | Cusabio |
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Description: Recombinant Human Neurofascin(NFASC),partial expressed in E.coli |
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Recombinant Neurofascin (NFASC) |
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| 4-RPL939Hu01 | Cloud-Clone |
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Description: Recombinant Human Neurofascin expressed in: E.coli |
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Human Neurofascin (NFASC) Protein |
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| 20-abx068240 | Abbexa |
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Neurofascin (NFASC) Polyclonal Antibody (Human) |
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| 4-PAL939Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC) |
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Neurofascin (NFASC) Polyclonal Antibody (Human), APC |
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| 4-PAL939Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with APC. |
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Neurofascin (NFASC) Polyclonal Antibody (Human), Biotinylated |
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| 4-PAL939Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with Biotin. |
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Neurofascin (NFASC) Polyclonal Antibody (Human), Cy3 |
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| 4-PAL939Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with Cy3. |
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Neurofascin (NFASC) Polyclonal Antibody (Human), FITC |
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| 4-PAL939Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with FITC. |
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Neurofascin (NFASC) Polyclonal Antibody (Human), HRP |
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| 4-PAL939Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with HRP. |
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Neurofascin (NFASC) Polyclonal Antibody (Human), PE |
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| 4-PAL939Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with PE. |
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Human Neurofascin (NFASC) ELISA Kit |
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| 20-abx152492 | Abbexa |
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Human Neurofascin (NFASC) CLIA Kit |
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| 20-abx495996 | Abbexa |
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Mouse Neurofascin (NFASC) ELISA Kit |
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| abx390014-96tests | Abbexa | 96 tests | EUR 911 |
Rat Neurofascin (NFASC) ELISA Kit |
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| abx391685-96tests | Abbexa | 96 tests | EUR 911 |
Human Neurofascin(NFASC) ELISA kit |
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| CSB-EL015743HU-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
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Description: Quantitativesandwich ELISA kit for measuring Human Neurofascin (NFASC) in samples from serum, plasma, cerebrospinalfluid (CSF), tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human Neurofascin(NFASC) ELISA kit |
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| 1-CSB-EL015743HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Neurofascin(NFASC) in samples from serum, plasma, cerebrospinalfluid(CSF), tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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Human Neurofascin (NFASC) ELISA Kit |
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| SEL939Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5189.65 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids. |
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Human Neurofascin (NFASC) ELISA Kit |
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| SEL939Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 515.03 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids. |
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Human Neurofascin (NFASC) ELISA Kit |
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| SEL939Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 692.9 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids. |
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Human Neurofascin (NFASC) ELISA Kit |
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| SEL939Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2818.05 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neurofascin (NFASC) in Tissue homogenates and other biological fluids. |
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Human Neurofascin (NFASC) ELISA Kit |
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| 4-SEL939Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neurofascin (NFASC) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
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Human Neurofascin(NFASC)ELISA Kit |
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| QY-E01656 | Qayee Biotechnology | 96T | EUR 394 |
Mouse Neurofascin, Nfasc ELISA KIT |
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| ELI-45737m | Lifescience Market | 96 Tests | EUR 865 |
Chicken Neurofascin, NFASC ELISA KIT |
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| ELI-15003c | Lifescience Market | 96 Tests | EUR 928 |
Human Neurofascin, NFASC ELISA KIT |
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| ELI-20708h | Lifescience Market | 96 Tests | EUR 824 |
Neurofascin (NFASC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAL939Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neurofascin (NFASC). This antibody is labeled with APC-Cy7. |
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ELISA kit for Human NFASC (Neurofascin) |
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| ELK6104 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A sandwich ELISA kit for detection of Neurofascin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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Rabbit Anti- Rat neurofascin (Nfasc)-phospor IgG (aff pure) |
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| AB-23249-A | Alpha Diagnostics | 100ug | EUR 482 |
Rat neurofascin (Nfasc)-control Control/blocking peptide |
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| AB-23249-CP | Alpha Diagnostics | 100ug | EUR 164 |
Rat neurofascin (Nfasc)-phosphor Control/blocking peptide |
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| AB-23249-P | Alpha Diagnostics | 100ug | EUR 164 |
Nfasc ELISA Kit| Mouse Neurofascin ELISA Kit |
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| EF015653 | Lifescience Market | 96 Tests | EUR 689 |
Nfasc ELISA Kit| Rat Neurofascin ELISA Kit |
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| EF019045 | Lifescience Market | 96 Tests | EUR 689 |
NFASC ELISA Kit| chicken Neurofascin ELISA Kit |
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| EF012420 | Lifescience Market | 96 Tests | EUR 689 |
Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-100ug |
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| QP6416-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-10ug |
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| QP6416-ec-10ug | EnQuireBio | 10ug | EUR 200 |
Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-1mg |
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| QP6416-ec-1mg | EnQuireBio | 1mg | EUR 1632 |
Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-200ug |
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| QP6416-ec-200ug | EnQuireBio | 200ug | EUR 634 |
Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-500ug |
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| QP6416-ec-500ug | EnQuireBio | 500ug | EUR 1060 |
Recombinant Human NFASC/ Neurofascin Protein, His, E.coli-50ug |
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| QP6416-ec-50ug | EnQuireBio | 50ug | EUR 263 |
Anti-NFASC antibody |
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| STJ24750 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. |
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Anti-Neurofascin (aa877-890) antibody |
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| STJ72603 | St John's Laboratory | 100 µg | EUR 359 |
Rabbit Polyclonal antibody Anti-CRBN |
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| Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
Neurofascin |
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| PR15066 | Neuromics | 50 ug | EUR 468 |
NFASC Antibody |
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| 31250-100ul | SAB | 100ul | EUR 252 |
NFASC Antibody |
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| 31250-50ul | SAB | 50ul | EUR 187 |
NFASC antibody |
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| 38553-100ul | SAB | 100ul | EUR 252 |
NFASC Antibody |
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| 1-CSB-PA073153 | Cusabio |
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Description: A polyclonal antibody against NFASC. Recognizes NFASC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
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NFASC Antibody |
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| 1-CSB-PA787178 | Cusabio |
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Description: A polyclonal antibody against NFASC. Recognizes NFASC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200 |
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NFASC Antibody |
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| DF7160 | Affbiotech | 200ul | EUR 304 |
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Description: NFASC Antibody detects endogenous levels of total NFASC. |
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NFASC Antibody |
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| ABD7160 | Lifescience Market | 100 ug | EUR 438 |
NFASC Conjugated Antibody |
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| C38553 | SAB | 100ul | EUR 397 |
NFASC Conjugated Antibody |
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| C31250 | SAB | 100ul | EUR 397 |
Nfasc/ Rat Nfasc ELISA Kit |
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| ELI-22181r | Lifescience Market | 96 Tests | EUR 886 |
NFASC siRNA |
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| 20-abx925794 | Abbexa |
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NFASC siRNA |
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| 20-abx925795 | Abbexa |
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NFASC siRNA |
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| 20-abx903541 | Abbexa |
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NFASC Peptide |
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| 42-393P | ProSci | 0.1 mg | EUR 338 |
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Description: (IN) NFASC Peptide |
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Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
NFASC Rabbit pAb |
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| A3053-100ul | Abclonal | 100 ul | EUR 308 |
NFASC Rabbit pAb |
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| A3053-200ul | Abclonal | 200 ul | EUR 459 |
NFASC Rabbit pAb |
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| A3053-20ul | Abclonal | 20 ul | EUR 183 |
NFASC Rabbit pAb |
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| A3053-50ul | Abclonal | 50 ul | EUR 223 |
NFASC Blocking Peptide |
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| DF7160-BP | Affbiotech | 1mg | EUR 195 |
Polyclonal Neurofascin (aa877-890) Antibody (internal region) |
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| AMM06631G | Leading Biology | 0.1 mg | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Neurofascin (aa877-890) (internal region). This antibody is tested and proven to work in the following applications: |
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Rat NFASC shRNA Plasmid |
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| 20-abx987343 | Abbexa |
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Mouse NFASC shRNA Plasmid |
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| 20-abx983017 | Abbexa |
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Human NFASC shRNA Plasmid |
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| 20-abx958008 | Abbexa |
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NFASC ELISA KIT|Human |
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| EF005338 | Lifescience Market | 96 Tests | EUR 689 |
NFASC ELISA Kit (Human) (OKCD01074) |
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| OKCD01074 | Aviva Systems Biology | 96 Wells | EUR 909 |
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Description: Description of target: Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.By similarity ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.115 ng/mL |
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NFASC ORF Vector (Human) (pORF) |
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| ORF026068 | ABM | 1.0 ug DNA | EUR 405 |
Nfasc ORF Vector (Mouse) (pORF) |
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| ORF051272 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Mouse) (pORF) |
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| ORF051273 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Mouse) (pORF) |
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| ORF051274 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Mouse) (pORF) |
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| ORF051275 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Rat) (pORF) |
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| ORF071257 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Rat) (pORF) |
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| ORF071258 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Rat) (pORF) |
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| ORF071259 | ABM | 1.0 ug DNA | EUR 506 |
Nfasc ORF Vector (Rat) (pORF) |
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| ORF071260 | ABM | 1.0 ug DNA | EUR 506 |
NFASC sgRNA CRISPR Lentivector set (Human) |
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| K1420901 | ABM | 3 x 1.0 ug | EUR 339 |
Nfasc sgRNA CRISPR Lentivector set (Rat) |
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| K7550101 | ABM | 3 x 1.0 ug | EUR 339 |
Nfasc sgRNA CRISPR Lentivector set (Mouse) |
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| K4184901 | ABM | 3 x 1.0 ug | EUR 339 |
NFASC sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K1420902 | ABM | 1.0 ug DNA | EUR 154 |
NFASC sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K1420903 | ABM | 1.0 ug DNA | EUR 154 |
NFASC sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K1420904 | ABM | 1.0 ug DNA | EUR 154 |
Nfasc sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7550102 | ABM | 1.0 ug DNA | EUR 154 |
Nfasc sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7550103 | ABM | 1.0 ug DNA | EUR 154 |
ilicicola pressure CBS 112217 (GenBank AY725686). To our information, that is the primary report of Calonectria and Cylindrocladium genera and the illness brought on by C. ilicicola from Iran. References: (1) R. Cheewangkoon et al. Persoonia 23:55, 2009. (2) P. W. Crous and M. J. Wingfield. Mycotaxon 51:341, 1994. (3) P. W. Crous et al. Stud. Mycol. 50:415, 2004.